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The figure shows the results of 1 of 3 independent experiments with similar results. (B) p 0. 005. TAGLN is a down stream regulated gene of HOXB7, HOXC6 and HOXC8 activity in VW MPSCsBecause inhibition of HOX genes expressed in VW MPSCs altered important functions of these cells as exemplified by in gel sprouting, we performed cDNA microarray analysis using Affymetrix DNA chips in order to identify potential down stream regulated genes of HOXB7, HOXC6 and HOXC8 activity (Fig. 3 and Supplementary Table S1 online). To Adidas Stan Smith Blue Navy this aim CD44(+) VW MPSCs were transfected with HO.

(A) Equal cell numbers of VW MPSCs were seeded and cultured in NGM and Adidas Stan Smith Women

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transfected with Control (non silencing) or HOXB7, HOXC6 and HOXC8, siRNA. After 4 days cell numbers were analysed. Data are presented as mean SD from 3 independent experiments performed in duplicates each. (B) For analysing the sprouting capacity transfected cells were embedded in GFR Matrigel as 3D spheroids. In Adidas Stan Smith US

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Adidas Stan Smith Blue Navy

Adidas Stan Smith Blue Navy

gel sprouting was quantified 48 hours after embedding. The data represent the mean cumulative length of all cord like sprouts growing from 10 individual spheroids per experimental group.

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with gene silencing for HOXB7, HOXC6 and HOXC8. Moreover, treatment of VW MPSCs after silencing of these HOX genes with angiogenic growth factors including VEGF, FGF2, PDGF, TGF1 did not restore the reduced in gel sprouting (not shown). Figure 2: Silencing over expressed HOX genes in cultured vascular wall derived MPSCs reduces in gel sprouting while proliferation is not affected.

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Adidas Stan Smith Blue Navy

Adidas Stan Smith Blue Navy

´╗┐Fig. S3A online). To further evaluate the functional consequences of HOXB7, HOXC6 and HOXC8 gene silencing we analysed the capacity of VW MPSCs to invade Matrigel (in gel sprouting) when these cells were cultured as spheroids following transfection with siRNAs specific for these HOX genes, either individually or in combination, and with control siRNA (Fig. 2B). The results show that VW MPSCs cultured in NGM alone and those transfected with control siRNA exhibit a higher invasive capacity in comparison to VW MPSCs Adidas Stan Smith Triple Black

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Adidas Stan Smith Blue Navy

Adidas Stan Smith Blue Navy

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