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they were not in G0.

(Fig. 6, Supplementary Fig. S5 online). As demonstrated by the proliferation marker Ki67 most of the cells were proliferating, meaning that Adidas Stan Smith Deconstructed

´╗┐OXC6 silencing was obtained at the protein level by Western blot analyses (Fig. 5B) and immunofluorescence staining of cultured VW MPSCs (Supplementary Fig. S3B online). Furthermore, the protein expression levels of calponin1 (CNN1), an additional marker for mature SMCs, were Adidas Stan Smith Gold Silver also enhanced in response to HOXB7, HOXC6 and HOXC8 silencing (Fig. 5B). Figure 5: Increased TAGLN expression in human VW MPSCs upon HOXB7, HOXC6, and HOXC8, and inhibition by siRNA treatment. (A) VW derived MPSCs were transfected with Control (non silencing) or HOXB7, HOXC6 and HOXC8, siRNA. After 4 days cells were harvested and subjected for mRNA isolation and qRT PCR quantification of the late SMC differentiation marker TAGLN. (B) Increased TAGLN as well as CNN1 expression were confirmed at the protein level after gene silencing of HOXB7, HOXC6 and HOXC8 in VW MPSCs.

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Adidas Stan Smith Gold Silver

(C, D) Silencing H1 mRNA expression directly decreases gene expression of TAGLN and CNN1. Data are presented as mean SEM from 4 independent experiments performed in duplicates each p 0. 05. Interestingly, silencing of these HOX genes also resulted in an up Adidas Stan Smith Peach Green

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Fur.

Adidas Stan Smith Gold Silver

Adidas Stan Smith Gold Silver

5C,), with the latter being confirmed at the protein level (Fig. 5D). This resulted in substantially reduced expression of the analyzed HOX genes (Fig. 5C). To further rule out that the changes in Histone gene expression observed upon silencing of the selected HOX genes may not simply be a consequence of affecting the cell cycle, we determined the stages of the cell cycle phases of cultured VW MPSCs upon silencing of HOX genes and H1 Adidas Stan Smith Vapour Pink

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Adidas Stan Smith Gold Silver

regulation of histone H1, both at transcriptional and translational levels (Supplementary, Fig. S3B online). Thus, we were curious to know whether H1 knockdown might affect TAGLN or CNN1 expression. Using specific siRNA we could effectively silence H1 in VW MPSCs (Fig. 5C) with concomitant reductions of TAGLN and CNN1 expression (Fig.

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Adidas Stan Smith Gold Silver

Adidas Stan Smith Gold Silver

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